Effects of Unripe Musa Paradisiaca on the Histochemistry of the Testis and Testosterone Levels in Adult Albino Rats

A S Alabi, G O Omotosho, CN B Tagoe, O B Akinola, B U Enaibe

Abstract


This study was aimed at determining the effects of the unripe fruit of Musa paradisiaca on the testis and
testosterone levels in male Wistar rats. The animals were grouped into three, comprising a control, and 2 treatment groups
administered with different doses (500 mg/kg and 1000 mg/kg) daily of the fruit flour over 28 days. Histochemical evaluation
of the testes was done using Haematoxylin and Eosin, Periodic acid Schiff’s (PAS) and Feulgen staining techniques, while
the serum and homogenised testicular tissue were evaluated for testosterone levels using Accu-Bind ELISA Kit. The testis
of the treated groups showed more rapidly dividing cells and more population of sperm cells compared to the control group,
and also showed more positivity for Feulgen staining and PAS reaction. Both serum and testicular testosterone levels were
however reduced. Serum testosterone was significantly lowered in the animals given the low dose (0.67 ± 0.03 ng/ml),
compared to those given high dose (0.85 ± 0.02 ng/ml) and the control animals (1.88 ± 0.15 ng/ml) (p < 0.05). Changes in
testicular testosterone were not statistically significant. The study suggests that M. paradisiaca fruit has reproductive
enhancing potential when consumed moderately, but this benefit may not be related to testosterone levels

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