Anti-hyperglycaemic Effects of Rutin on Blood Glucose, Oxidative Stress Biomarkers and Lipid Peroxidation in Alloxaninduced Hyperglycaemic Wistar Rats

Y Tanko, A I Salisu, K A Mohammed, S A Musa, A Jimoh, R Yusuf

Abstract


The present study investigated the anti-hyperglycaemic effect of rutin on blood glucose, oxidative stress
biomarkers and lipid peroxidation in alloxan induced hyperglycaemic wistar rats. Diabetes was induced in rats by an
intraperitoneal (i.p) injection of alloxan monohydrate 150 mg/kg body weight. Twenty five wistar rats were divided as
follows; Group1 served as diabetic control received distilled water 2 mg/kg, Group served as positive control received 2
mg/kg glibenclamide, 3, 4 and 5 received rutin at 50, 100 and 200 mg/kg body weight for 28 days respectively. At the end
of the treatment, rats were sacrificed and the blood and serum were used for the analysis of blood glucose and oxidative
stress biomarkers respectively. The determinations of blood glucose levels were carried out at intervals of 7, 14, 21 and 28
days respectively Serum oxidative stress biomarkers lipid peroxidation, were done on the 28 days. Administrations of rutin
at the three different doses 50,100 and 200 mg/kg to diabetic rats significantly (p<0.05) decreased the blood glucose levels
as compared to diabetic control. The dose of 200 mg/kg exhibited a maximum glucose lowering effect with blood glucose
of 102.8± 0.06 as compared to diabetic control 346.2±0.16. Furthermore, in relation to the oxidative stress biomarkers there
was a significant (p<0.05) increased in the levels of gluthathione peroxidase, superoxide dismutase and catalase as compared
to control. However, there was also a significant decreased in the malondialdehyde levels as compared to control. It may be
concluded that oral administration of Rutin for 28 days decreases blood glucose levels and prevented oxidative stress and
antioxidant status in hyperglycaemic rats

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