High Reticulocyte Count with Abnormal Red Blood Cell Morphology in Normal Wistar Rats after Garlic Administration

H A Salami, M A Tukur, A Bukar, I A John, A Abubakar, J Jibrin

Abstract


Despite the high acceptability of Allium sativa (Garlic) as a remedy for many diseases as earlier stated by many
researchers, previous studies have shown that chronic and unregulated consumption of garlic may result to intra vascular
haemolytic anaemia in rats. The present study was conducted to examine the effect of crude extract of garlic on microscopic
status of red blood cells and some other haematological indices of normal albino rats. The animals were grouped into two;
group 1 were normal animals treated with water while group 2 were normal animals administered 150mg/kg body weight of
crude extract of garlic on alternate days for three weeks. At the end of three weeks treatment, blood samples obtained from
the tail vein of the rats were used for haematological indices and erythrocyte morphology. The values obtained were
expressed as Mean± SEM and compared using student t test. The results showed that there was no significant difference in
the PCV which was 43.20 ± 0.80% and 45.00 ± 0.36% in both control and experimental groups respectively. However, the
RBCs were significantly decreased (P< 0.05) from 166.80 ± 3.44 x106 µL-1 in the control group to 87.80 ± 9.34 x106 µL-1 in
the treatment group. The percentage reticulocyte counts on the other hand significantly increased from 2.60±4.25% in control
group to 11.20± 16.4% in treated group. Fragmented RBCs with a lot of schistocytes with adequate platelets were seen on
peripheral blood film of crude garlic treated rats as compared to control. Our results suggested intravascular haemolysis and
numerous reticulocytes on blood film confirmed our view on bone marrow response. The presence of schistocytes and
acanthocytes may be an indication that the liver is involved in the observed effect.


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